Browsing Research articles (Dept of Biological Sciences) by Author "Mubyana-John, T."
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Siele, M.P.; Mubyana-John, T.; Bonyongo, M.C. (Global Science Books http://www.globalsciencebooks.com/index.php?page=shop.product_details&category_id=1&flypage=shop.flypage&product_id=5&option=com_virtuemart&Itemid=56&vmcchk=1&Itemid=56, NaN, 2008)[more][less]
Abstract: Soil cover influences soil biological and chemical processes in various ways. The effects of soil cover (bare soil without litter, litter cover, Mopane tree canopy and grass cover) on soil respiration and microbial population at four sites in the Mopane woodland of north western Botswana were investigated. Soil respiration rates were measured using an infrared gas analyzer. Nitrifying bacterial populations were quantified by MPN on ammonium and nitrite calcium carbonate media, oligotrophs on 1% nutrient agar, actinomycetes on starch casein agar, bacteriovorous protozoans by MPN on sodium chloride agar baited with Enterobacter, and fungal biomass carbon using buried slides were also determined. Soil respiration in different habitats was significantly influenced by type of cover, diurnal temperature variations and soil moisture. Soils under cover showed significantly higher soil respiration than the bare soils. Autochthonous bacterial populations in the Mopane woodland soils consisted mostly of oligotrophs which varied insignificantly between seasons. Fungi were the most dominant microorganisms in all the Mopane woodland soils, with biomass of 162.05 to 282.14 μg C/g soil in the wet season and 11.84 to 44.16 μg C/g soil in the dry season. Microscopic observation of buried slides revealed that fungi play a vital role in holding soil particles together in these soils. The results showed a strong positive relationship between changes in fungal biomass and fluctuation of soil respiration. However, no clear correlation was observed between the variation of soil respiration and other microbial populations (oligotrophic bacteria, actinomycetes, and nitrifying bacteria). Overall these results indicate that in soils under Mopane tree canopy, fungi contribute significantly to soil structure and soil respiration. Other microbial populations consist mostly of oligotrophs which show minimal seasonal variations. Soil moisture significantly influences seasonal fluctuation in soil respiration. URI: http://hdl.handle.net/10311/292 Files in this item: 1
The Effects of Soil Cover.pdf (6.625Mb) -
Mubyana-John, T.; Wutor, V.C.; Yeboah, S.O.; Ringrose, S. (Academic Journals. http://www.academicjournals.org/SRE, NaN, 2007)[more][less]
Abstract: The influence of wild fires on microbial community structure, soil organic matter, sulphur oxidising and nitrifying microbial populations in the floodplains of the Okavango Delta of Botswana was assessed. Microbial community structure was assessed by phospholipids ester-linked fatty acids (PLFA) quantification while microbial sulphur oxidisers were assessed by Most Probable Number (MPN). Community structure assessment showed that burning shifted the microbial community structure from single cellular bacteria being the dominant groups to filamentous fungi and actinomycetes being the most dominant groups. Generally burning increased the fungal component (18:2 w6) matrix from 3.40 to 8.35 while the actinomycetes and sulphur reducing bacterial (10 Me 16:0) component also increased from 1.02 to 1.70 mostly in the floodplains. Generally, the organic matter content declined with burning. However, the influence of burning on soil pH was non conclusive. Soil microbial biomass carbon increased slightly after the fire. The number of heterotrophic and nitrite-oxidizing and sulphur reducing bacteria increased. Overall, these results indicate that burning significantly alters the microbial community structure as large above ground losses of nutrients during and after burning often results in low quantities of nutrients released into the soil. URI: http://hdl.handle.net/10311/349 Files in this item: 1
Fire and.pdf (2.461Mb) -
Mubyana-John, T.; Wright, M.G. (Global Science Books, http://www.globalsciencebooks.info/, NaN, 2011)[more][less]
Abstract: A study assessing fungi isolated from three climatic regions of Botswana as a possible control for root knot nematodes (Meloidogyne spp.) affecting the tomato(Lycopersicon esculentum L. var. ‘Money maker’) crops was conducted. Out of 1,250 soil fungal isolates, only 232 soil fungi from the Okavango Delta, Bobirwa district and different saltpans in Botswana could be sub cultured, and screened for antagonism against Meloidogyne sp. Seven of the isolates i.e., Trichoderma sp., Penicillium sp., Dendriphiopsis sp., Fusarium chlamydosporium, Cochliobolus sativus, Aspergillus fumigatus and an unidentified sterile fungus showed potential to immobilize nematode juveniles on agar plates within 2-4 days. C. sativus and Dendriphiopsis sp. showed maximum paralysis on agar plates and eventually death of the Meloidgyne sp. juveniles. In greenhouse studies using tomato plants the fungi alleviated the effect of nematodes by increasing plant, shoot height and root weight as compared to the reference controls. Cochliobolus sativus and Trichoderma sp. showed maximum plant protection of tomato plants under greenhouse conditions. Fungi ability to produce cellulase and chitinase were some of the mechanisms studied. The results indicated that Trichoderma sp., Penicillium sp., Dendriphiopsis sp., Fusarium chlamydosporium, Cochliobolus sativus and Aspergillus fumigatus are nematode antagonistic fungi indigenous to Botswana that can be used to control nematodes as they are better adapted in comparison to introduced fungi. URI: http://hdl.handle.net/10311/917 Files in this item: 1
Mbuyana-John_AJPSB_2011.pdf (813.1Kb) -
Banda, A.; Mubyana-John, T.; Taylor, J.E. (Global Science Books, http://www.globalsciencebooks.info/, NaN, 2011)[more][less]
Abstract: The influence of burning on soil microbial dehydrogenase activity, nitrogen content and fungal population along the Boro route in the Okavango Delta was assessed in the flood and dry seasons. Soil samples from the burnt plots and the adjacent control un-burnt plots were cultured on agar plates using dilution methods. Fusarium spp. were dominant while Aspergillus species were low in burnt plots. The other fungi such as Drechslera sp., Exophiala jeanselmmei, Penicillium compactum and Chrysosporium merdarium were only in the burnt plots as compared to unburnt control plots. However, fungal diversity and soil dehydrogenase activity reduced after 6 months of burning showing significant increase in Chrysosporium merdarium in almost all the burnt plots. The influence of burning on soil nitrogen was insignificant instead flooding had a stronger influence on nitrogen content than burning. The results indicate that burning increased fungal diversity and biomass, however, reduces overall microbial enzyme activity after burning without influencing soil nitrogen and pH. URI: http://hdl.handle.net/10311/919 Files in this item: 1
Mbuyana_AJPSB_2011.pdf (220.0Kb)
Now showing items 1-4 of 4