Matsheka, M.I.; Lastovica, A.J.; Elisha, B.G.(American Society for Microbiology. http://jcm.asm.org/, NaN, 2001)
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Abstract:
A 1.6-kb DNA fragment isolated from a Campylobacter concisus genomic library gave C. concisus-specific restriction
fragment length patterns when it was used as a probe in hybridization studies. All of the strains tested,
including type strains and clinical isolates, contained a 0.5-kb HindIII fragment that hybridized to the probe.
DNA sequencing of the 1.6-kb fragment identified three open reading frames (ORFs). One of the ORFs encodes
the carboxy terminus of GyrB, and the translational products of ORF2 and ORF3 showed similarity to hypothetical
proteins, previously identified in Campylobacter jejuni. DNA-DNA hybridization studies with a fragment
internal to ORF3 showed that this sequence was responsible for the signal observed with the 0.5-kb HindIII
fragment. A rapid PCR assay was developed and evaluated. Primers that annealed to the extremities of the 1.6-kb
fragment were used to obtain an amplicon of the correct size from both reference and clinical strains of C. concisus.