Show simple item record

dc.contributor.author Moyo, S.
dc.contributor.author Gashe, B.A.
dc.contributor.author Collison, E.K.
dc.contributor.author Mpuchane, S.
dc.date.accessioned 2008-10-09T09:23:14Z
dc.date.available 2008-10-09T09:23:14Z
dc.date.issued 2003
dc.identifier.citation Moyo, S. et al (2003) Optimising growth conditions for the pectinolytic activity of Kluyveromyces wickerhamii by using response surface methodology, International Journal of Food Microbiology, Vol. 85, pp. 87-100 en
dc.identifier.issn 0168-1605
dc.identifier.uri http://hdl.handle.net/10311/228
dc.description.abstract This present study was undertaken to find optimum conditions of pH, temperature and, period of incubation for the pectinolytic activity of Kluyveromyces wickerhamii isolated from rotting fruits and to assess the effect of these factors by use of response surface methodology (RSM). A central composite rotatable design was used as an experimental design for the analysis of the allocation of treatment combinations. A second order polynomial regression model was fitted and was found adequate, with an R2 of 0.94469 ( P < 0.001). The effects of temperature and pH were the most significant factors in influencing enzyme production. Estimated optimum conditions were as follows: pH 5.0, temperature, 32 jC and an incubation period of 91 h. Pectinesterase (PE), pectin lyase (PL), and cellulase activities were not detected. Pectinase production was partially constitutive. Pectin was degraded by the isolated strain of K. wickerhamii in the current study, and the pectinolytic activity is referred to as polygalacturonase (PG) activity. Crude enzyme extract was thermostable at various temperatures and, stimulated by the presence of Ca2 + ions but inhibited by other ions like Mg2 +, Zn2 +, Co2 +, Mn2 + and Na+. en
dc.language.iso en en
dc.publisher Elsevier / www.elsevier.com/locate/ijfoodmicro en
dc.subject Kluyveromyces wickerhamii en
dc.subject Pectinase en
dc.subject Polygalacturonase en
dc.subject Response surface methodology en
dc.title Optimising growth conditions for the pectinolytic activity of Kluyveromyces wickerhamii by using response surface methodology en
dc.type Article en


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search UBRISA


Advanced Search

Browse

My Account