Browsing Faculty of Science by Author "Etoa, F."
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Kuete, V.; Ngameni, B.; Tsafack, A.M.; Ambassa, P.; Simo, I.K.; Bezabih, M.; Etoa, F.; Ngadjui, BT; Abegaz, B.M.; Beng, V.P. (Pharmacologyonline. http://www.pharmacologyonline.org/, NaN, 2007)[more][less]
Abstract: The crude extract from the twigs of Dorstenia elliptica (DET) as well one of the five compounds isolated from this extract namely O-[3-(2, 2-dimethyl-3-oxo-2H-furan-5-yl)-3- hydroxybutyl]bergaptol (5) were tested for their antimicrobial activity against bacteria and fungi. The Agar disc diffusion test was used to determine the sensitivity of the tested samples while the well micro-dilution was used to determine the minimal inhibition concentrations (MIC) and the minimal microbicidal concentration (MMC) of the active samples. The results of the disc diffusion assay showed that DET prevented the growth of all the 22 tested microbial species while the two tested compounds showed selective activity. At the tested MIC interval ranged from 1.22 to 156.25 μg/ml for the crude extracts and 0.08 to 39.06 μg/ml for the compounds, the activity of DET was noted on 20 of the 22 microbial species. The antimicrobial activities of DET as well as that compound 5 are being reported for the first time. The overall results provide promising baseline information for the potential use of the crude extract in the treatment of bacterial and fungal infections. URI: http://hdl.handle.net/10311/287 Files in this item: 1
ANTIMICROBIAL ACTIVITY OF THE.pdf (4.398Mb) -
Kuete, V.; Metuno, R.; Ngameni, B.; Tsafack, A.M.; Ngandeu, F.; Fotso, G.W.; Bezabih, M.; Etoa, F.; Ngadjui, B.T.; Abegaz, B.M.; Penlap, B.V. (Elsevier Ireland Ltd. www.elsevier.com/locate/jethpharm, NaN, 2007)[more][less]
Abstract: The crude extract from Treculia obovoidea was subjected to purification by repeated chromatography. Eight compounds were isolated from Treculia obovoidea and identified as Psoralen (1), Bergapten (2), 7-methoxycoumarin (3), 7-hydroxycoumarin (4), 4,2 ,4 -trihydroxychalcone (5), 4,2 ,4 -trihydroxy-3-prenylchalcone (6), 3-hydroxy-4-methoxybenzoic acid (7) and O-[3-(2,2-dimethyl-3-oxo-2H-furan-5-yl) butyl] bergaptol (8). These compounds together with the extract were tested for their antimicrobial activity against Gram-positive bacteria (six species), Gram-negative bacteria (12 species) and three Candida species using micro-dilution methods for the determination of the minimal inhibition concentration (MIC) and the minimal microbicidal concentration (MMC). The MIC values obtained with the crude extracts varied from 78.12 to 156.25 g/ml against 17 (80.95%) of the 21 tested microorganisms. All the isolated compounds showed selective activity. The antimicrobial activity of this plant as well as that of compounds 6 and 8 is being reported for the first time. The obtained results provide promising baseline information for the potential use of these crude extract as well as some of the isolated compounds in the treatment of bacterial and fungal infections. URI: http://hdl.handle.net/10311/288 Files in this item: 1
Now showing items 1-2 of 2