Show simple item record

dc.contributor.author Mpoloka, S.W.
dc.contributor.author Kgotlele, T.
dc.contributor.author Wally, A.
dc.date.accessioned 2012-05-10T08:37:25Z
dc.date.available 2012-05-10T08:37:25Z
dc.date.issued 2008-04-17
dc.identifier.citation Mpoloka, S.W. et al (2008) Determination of allele frequencies in nine short tandem repeat loci of five human sub-populations in Botswana, African Journal of Biotechnology, Vol. 7 no. 8, pp. 1023-1027 en_US
dc.identifier.issn 1684–5315
dc.identifier.uri http://hdl.handle.net/10311/1010
dc.description.abstract Allele frequencies for nine short tandem repeat (STR) loci from the AmpFlSTR® Profiler Plus PCR Amplification Kit were determined in five subpopulations across Botswana and tested for suitability for use in individual identification. DNA was extracted from whole blood samples collected from 150 unrelated individuals. Targeted regions of DNA (vWA, FGA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D18S51, D21S11 and the sex determining locus Amelogenin) were amplified using multiplexed PCR. The alleles showed a high degree of polymorphism, with heterozygosity above 67%, indicating that there is no significant inbreeding within the subpopulations. Matching probability was below 0.2 hence power of discrimination was high, indicating that the alleles from the five subpopulations can be used in human identifications. The present study is the first reported attempt at determining allele frequencies of subpopulations in Botswana and could possibly be used in developing a national DNA database. en_US
dc.language.iso en en_US
dc.publisher Academic Journals, www.academicjournals.org en_US
dc.subject DNA typing en_US
dc.subject short tandem repeat (STR) en_US
dc.subject polymerase chain reaction en_US
dc.subject allele frequency en_US
dc.subject Botswana en_US
dc.title Determination of allele frequencies in nine short tandem repeat loci of five human sub-populations in Botswana en_US
dc.type Published Article en_US
dc.link http://www.academicjournals.org/AJB en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search UBRISA


Advanced Search

Browse

My Account