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Investigating evidence for UV-B induced mutagenesis in dimorphotheca sinuata using the 18S rDNA and rbcL gene sequences

Mpoloka, S.W. (American-Eurasian Network for Scientific Information. http://www.aensi.org/aeb_online.html, NaN, 2008)

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Abstract:

The nuclear 18S rDNA and chloroplast encoded rbcL gene sequences were used to investigate the evidence of residual UV-B induced mutagenesis in Dimorphotheca sinuata plants. The probes were generated by the polymerase chain reaction and labelled with a non-radioactive Digoxigenin label and used in Southern hybridization studies. High levels of variability in the 18S rDNA gene were found, pointing to genome rearrangements and possibly genome instability due to UV-B effects.

URI:

http://hdl.handle.net/10311/484

Files in this item: 1

Investigating evidence.pdf (1.233Mb)

Residual effects of prolonged UV-B exposure on photosynthetic gene expression

Mpoloka, S.W. (Academic Journals, www.academicjournals.org, August 18, 2010)

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Abstract:

Residual long-term effects of increased UV-B radiation were investigated in an indigenous plant species, Dimorphotheca sinuata, by analysing photosynthetic gene expression. Reductions were observed in the amount of the rbcL and psbA mRNA expressed in progeny of plants that were previously subjected to enhanced UV-B levels. However, observed reductions did not attain statistical significance. This could explain the reduction in net biomass and physiological and biochemical parameters observed by other researchers as a result of UV-B exposure. Results from this study with plants grown in the absence of UV-B point to changes in the regulation of photosynthetic genes and such mutations due to raised UV-B levels could cause permanent changes in plant populations.

URI:

http://hdl.handle.net/10311/1011

Files in this item: 1

Mpoloka SRE 2010.pdf (122.9Kb)

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Browsing by Subject "UV-B"

Browsing by Subject "UV-B"

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