Browsing by Subject "Glutathione"
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Chaturvedi, P.; Pipedi-Tshekiso, M.; Moseki, B.; Kwape, P.E. (Academic Journals, http://www.academicjournals.org/SRE, NaN, 2011)[more][less]
Abstract: Bark of Bauhinia purpurea tree was collected and both the water and methanol extracts were prepared. Both extracts were tested for free radical scavenging activities and phenol content. Water extract was then tested for its protective potentials against alcohol induced toxicity. For this experiment, twenty rats were randomly divided into four groups, alcohol control group, normal control and two experimental groups. The rats in alcohol control group received alcohol (5 g/kg), rats in normal control were given water. Rats in two experimental groups were administered two doses of methanol extract (50 and 100 mg/kg respectively) plus alcohol (5 g/kg) for 30 days. At the end of the experiment, rats were killed after ether anesthesia. Liver and blood samples were collected for biochemical measurements. Parameters measured were thiobarbituric acid reactive substances, serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, reduced glutathione, glutathione peroxidase, superoxide dismutase, catalase and ascorbic acid. Results showed that both methanol and water extracts scavenged free radicals equivalent to gallic acid scavenging and were found rich in total phenol content. Results of in vivo experiments showed that the water extract inhibited lipid peroxidation, protected the experimental animals from hepatic toxicity and maintained the levels of antioxidants in dose dependent manner. URI: http://hdl.handle.net/10311/1018 Files in this item: 1
Chaturvedi_SRE_2011.pdf (207.7Kb) -
Chaturvedi, P. (Oxford University Press, Oxford Journals; http://ecam.oxfordjournals.org/, March NaN, 2008)[more][less]
Abstract: In the present study, inhibitory effect of the methanol extract of Raphanus sativus root on lipid peroxidation has been carried out in normal rats. Graded doses of methanol extract of root of the plant (40, 80 and 120 mg kg 1 body weight) were administered orally for 15 days to experimental treated rats. Distilled water was administered to experimental control rats. At the end of experiment, rats were killed by decapitation after ether anesthesia. Blood and liver were collected to measure thiobarbituric acid reactive substance, reduced glutathione and activity of catalase. Results indicated that the extract of R. sativus root reduced the levels of thiobarbituric acid reactive substance significantly in all experimental treated groups (P50.05) as compared to the experimental control group. It also increased the levels of reduced glutathione and increased the activity of catalase. In vitro experiments with the liver of experimental control and experimental treated rats were also carried out against cumene hydroperoxide induced lipid peroxidation. The extract inhibited in vitro cumene hydroperoxide induced lipid peroxidation. R. sativus inhibits lipid peroxidation in vivo and in vitro. It provides protection by strengthening the antioxidants like glutathione and catalase. Inclusion of this plant in every day diet would be beneficial. Description: eCAM Advance Access originally published online on February 20, 2007 eCAM 2008 5(1):55-59; doi:10.1093/ecam/nel077 Version available here is the 2007 version. URI: http://hdl.handle.net/10311/182 Files in this item: 2
Inhibitory response of raphanus.pdf (861.5Kb)license.txt (1.998Kb) -
Chaturvedi, P.; George, S.; John, A. (British Journal of Biomedical Science. http://www.ibms.org/index.cfm?method=publications.british_journal, NaN, 2007)[more][less]
Abstract: In the present study, preventive and protective effects of Ocimum gratissimum in ethanol-induced hepatotoxicity are assessed in albino rats. A methanol extract of O. gratissimum leaves is prepared, with a yield of 3.5% (w/w) of the dry weight of leaves. Graded doses of the extract (10, 20, 40 and 80 mg/kg body weight), together with ethanol (5 gm/kg body weight) are administered orally to experimental groups for 30 days. Normal control rats receive distilled water only, while rats in an alcohol control group (AC) receive ethanol only for 30 days. O. gratissimum reduced the level of thiobarbituric acid reactive substance in all experimental groups (E1â E4). Alanine transaminase and aspartate transaminase levels fell in all experimental groups (E1â E4), but this reduction was significant only in groups E3 and E4 (P<0.05), indicating inhibition of lipid peroxidation by free radicals generated after ethanol metabolism. Levels of antioxidants also increased. Ascorbic acid and glutathione levels increased in all experimental groups (E1â E4; P<0.05 and P<0.01, respectively). A significant increase in catalase (P<0.05) was noted only in group E4, although an upward trend was noted in all experimental groups. This study shows that O. gratissimum prevents free radical damage to the liver and thus protects the organ from oxidative stress. URI: http://hdl.handle.net/10311/309 Files in this item: 1
Preventive and pro.pdf (2.493Mb)
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